WP7

Cellular/molecular pharmacology. In vivo evaluation of the monomer-monomer interface inhibitors against enzymes biolibrary and wt and resistant ovarian carcinoma cell line.

1. Enzyme bio-library preparation, enzyme purification.

2. Compounds library rapid screening assay.

3. Examination of cytotoxic effects of compounds designed to limit the intracellular levels of TS protein in cDDP-sensitive and cDDP-resistant cell lines and in a non-carcinoma cell line.

4. Evaluation of cell growth effects of TS monomer-monomer interface inhibitors in combination with cisplatin.

5. Characterization of the intracellular molecular mechanisms involved in the action of inhibitors.

6. Evaluation of transcription levels of the folate dependent enzymes in ovarian human cell lines (ATCC).

7. Identification of molecules competing with TS for their binding to mRNA.


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