WP6

SH-labelling and ligand-protein interaction studies

1. Identification of the highest affinity hits and low affinity ligands in solution. Protein (monomeric form/dimeric form-wt and mutant) fishing out of the best affinity inhibitor.

2. Structural characterization of protein-ligand complexes with compounds. SH-labelling from WP6 for structure-based drug design. Covalent and non covalent complexes.

3. Characterization of the energetics of the interaction small molecule hit-protein, lead-protein (TS monomeric/dimeric form-wild type and mutant).

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